Ask your proteome
a new question.
Any time you like.
Up to twenty samples and forty-eight targets. Each run.
Send us your prepared lysates, using the kit we provide, and we return publication-ready, quantified protein data in weeks. Every target resolved by molecular weight, on real bands you already know how to read.
Now accepting samples
The 3D-NPC
membrane
See how it works →
Traditional western blot membranes are one-and-done. After antibody probing, if you want more information from your sample, you have to run another blot.
In HyperBlot, the 3D-NPC membrane binds proteins covalently inside capillary nanopores, so they can be probed, imaged, regenerated and re-probed indefinitely.
Your blot is essentially immortalized.
Gather as much proteomic information from your samples as you want, limited only by the antibodies available, and because HyperBlot is open architecture, almost any commercially available antibody can be used in the system.
- Covalent capture
- Unlimited re-probing
- Fast, flow-through kinetics
- Any antibody, any vendor
Signaling cascade resolved in a single experiment
Performed on HEK cells treated with GPCR & RTK agonists. Probing with phospho-antibodies revealed the distinct signaling pathways downstream of each receptor. The entire experiment took two days, including every blot, all on the same membrane.
Twenty-one of those antibodies are shown here, grouped by pathway and labeled with their supplier catalog numbers: vehicle and six agonists, at 5 min, 30 min and 3 h.
Presented at the 2026 Gordon Research Conference, “Phosphorylation and G-Protein Mediated Signaling Networks.”
The readout is familiar on purpose.
For forty-five years the western blot has been the assay scientists believe. Slow, manual, temperamental, one target at a time, and still the thing a reviewer asks for when the result actually matters. We had no interest in asking you to give that up.
So HyperBlot returns bands. Real bands, resolved by molecular weight in SDS-PAGE, quantified the way you'd quantify them yourself. That’s deliberate. A platform that hands back numbers alone gets confirmed by western anyway, and research slows.
What changed is not the readout. It is what happens to the sample.
A traditional western is a question you ask once. The membrane is consumed, the aliquot is spent, and the next question costs another blot. HyperBlot doesn't consume the sample. Proteins bind covalently into the 3D-NPC membrane, so the membrane survives the first interrogation: as many targets as you want now, and more whenever you come back, against all of your original samples, with no new material and no batch effect between the answers.
That is not a faster western blot. It is a different unit of experiment. Your samples stop being consumables and become a queryable archive, a specimen you can keep interrogating as the hypothesis moves, for as long as the biology stays interesting.
A western blot is an experiment — run once, consumed, done. HyperBlot archives your specimen so you can keep interrogating it.
It matches the gold standard. Then it keeps going.
Identical samples and identical antibodies, compared across systems.
Same samples, same antibodies, same answer
It reads the same as a western
And it still reads that way a month later
Where conventional membranes stop, this one keeps going
Bottom: relative signal against cycle count, for a phosphosite and a housekeeping protein. Nitrocellulose and PVDF, stripped by any of four conventional protocols, fall to baseline within two or three cycles. HyperBlot holds past forty.
HyperBlot is validated in independent academic and industry laboratories, with results accepted for publication in a peer-reviewed journal. The platform accepts antibodies from any source, just like a traditional western, and we add to the list of validated supplier antibodies continually. A line co-developed with our preferred antibody partner runs on it out of the box. Pick your targets →
Provided for every submitted experiment
Images, figures, numbers, raw data files, and statistics in the form you would have produced yourself.
Publication-ready images
Individual raw images of every blot, plus multiplexed, color-resolved digital blot images ready to drop straight into a figure.
Four targets from one membrane, plus every target overlaid — click any to enlarge.
Signal quantitation and precise molecular-weight ID
A tagged MW marker in the loading buffer standardizes and aligns every lane, on every cycle.
The same bands, labeled two ways: quantified signal, or measured molecular weight. Click either to enlarge.
Statistics and graphs
Auto-generated statistics, lane traces, and figures.
Quantified per lane, then compared across conditions with p-values and fold changes. Click either to enlarge.
Access to the ProteoWise analysis tool
User-configurable data normalization, heat maps, dendrograms, and statistical comparison across every antibody and every condition in your experiment.
Normalize, sort by significance or fold change, and cluster antibodies and conditions against each other. Click either to enlarge.
Your membrane, archived
Stored desiccated, cataloged, and ready to re-probe whenever you want, with no new sample and no new shipment. Returned to you on request.
Talk to one of our specialists before ordering
Most researchers arrive with the same questions: can you really read unlimited targets from one sample, over time; which proteins can you detect, and at what sensitivity; and can you really keep my samples so I can come back and run new queries against them later?
We'd love to discuss HyperBlot Services with you. If it's a fit, we can talk through sample prep, what's detectable, and what the archive means for your project. When you're ready, we'll set up your account and send a prep kit, and we'll do the rest.
